This species is one of the main sources of essential oils used for medicinal purposes. There are two major types of lung cancer accounting for approximately 85% of all lung cancers: small cell lung cancer and non-small-cell lung cancer (NSCLC). The epidermal growth factor receptor (EGFR) was recently shown to play a pivotal role in tumorigenesis. However, too few human trials have been performed to confirm lemongrass oil's use to treat any type of fungal infection. Self-treating a chronic condition and avoiding or delaying standard care may have serious consequences. A549 cells were plated in 6 cm plates and allowed to attach for 24 h and then treated with or without essential oils for 48 h. Only adherent cells were harvested and then washed twice with ice-cold PBS. U.S. Food And Drug Administration. In Vietnam, 22,000 people are diagnosed with lung cancer and 19,500 die from the disease yearly. The cells were cultured at 37 C in RMPI1640 medium supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin in a 5% CO2 incubator. View abstract. Copyright © 2020 Duong Thu Trang et al. Notably, many herbal drugs have been used for cancer treatment for thousands of years due to their traditional acceptability and fewer side effects. Code of federal regulations-title 21, part 182-substances generally recognized as safe. A skin patch test should be done before using this or any essential oil. Helium was the carrier gas with a flow rate of 1.0 mL/min. The cells were stained with Hoechst 33342 solution (2′-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5′-bi-1H-benzimidazole trihydrochloride trihydrate) at a concentration of 5 μg/mL at 37°C for 30 min in the dark. Mix into a … 2013;88(3):381-5. doi:10.1590/abd1806-4841.20131800, Goes T, Ursulino F, Almeida-Souza T, Alves P, Teixeira-Silva F. Effect of lemongrass aroma on experimental anxiety in humans. After fixed gently in ice-cold 70% EtOH and incubated for 2 h at −4°C, cells were washed with ice-cold PBS twice. The essential oil from C. citratus is known as lemongrass oil with reported antimicrobial and anti-inflammatory properties. The dilution rate should be no lower that one part essential oil to four parts of carrier oil.. 2011;2(1):3-8. doi:10.4103/2231-4040.79796, Dhifi W, Bellili S, Jazi S, Bahloul N, Mnif W. Essential oils' chemical characterization and investigation of some biological activities: a critical review. The cellular proliferation of this panel of four NSCLC cell lines was examined by MTT assay over a 48 h period in the presence of increasing concentrations of various C. citratus essential oils. 2017;4:107-111. doi:10.1016/j.biopen.2017.03.004, Dany S, Mohanty P, Tangade P, Rajput P, Batra M. Efficacy of 0.25% lemongrass oil mouthwash: a three arm prospective parallel clinical study. RESULTS: Major constituents of C. citratus were geranial and neral, while A. cepa presented dipropyl disulfide and dipropyl trisulfide. BACKGROUND: Cymbopogon citratus and Allium cepa essential oils (EOs) are rich in terpenes and sulfur compounds respectively, both with antibacterial activity and different cell targets, supporting the idea that their combination can increase their efficacy. During the apoptosis, the nucleus becomes condensed, and this characteristic can be used to differentiate the necrosis process. A549 cells were treated with different concentrations (0, 1, and 10, Advanced Center for Bio-Organic Chemistry, Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam, Viettri University of Industry, Tien Kien, Lam Thao, Phu Tho, Vietnam, University of Science and Technology of Hanoi, VAST, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam, Center for Research and Technology Transfer, VAST, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam, V. S. Gadepalli, S. P. Deb, S. Deb, and R. R. Rao, “Lung cancer stem cells, p53 mutations and MDM2,”, P. Zarogoulidis, K. tsirgogianni, K. Darwiche et al., “EGFR-TKIs in adjuvant treatment of lung cancer: to give or not to give?”, K. Bencardino, M. Manzoni, S. Delfanti, A. Riccardi, M. Danova, and G. R. Corazza, “Epidermal growth factor receptor tyrosine kinase inhibitors for the treatment of non-small-cell lung cancer: results and open issues,”, A. K. Handique and H. B. Singh, “Antifungal action of lemongrass oil on some soil-borne plant pathogens,”, Z. T. Pinto, F. F. Sánchez, A. R. d. Santos et al., “Chemical composition and insecticidal activity of, K. Koba, K. Sanda, C. Guyon, C. Raynaud, J.-P. Chaumont, and L. Nicod, “In vitro cytotoxic activity of, S. Kpoviessi, J. Bero, P. Agbani et al., “Chemical composition, cytotoxicity and in vitro antitrypanosomal and antiplasmodial activity of the essential oils of four, M. Saleem, N. Afza, M. A. Anwar, S. M. A. Hai, and M. S. Ali, “A comparative study of essential oils of, P. Vyshali, K. J. T. Saraswathi, and G. R. Mallavarapu, “Chemical composition of the essential oils of, B. K. Matouschek and B. E. Stahl, “Phytochemical study of non volatile substances from, K. H. Miean and S. Mohamed, “Flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plants,”, H. T. K. Van, N. M. Quy, D. T. V. Ha et al., “Chemical composition and cytotoxic activity of the essential oils of, M. M. Blanco, C. A. R. A. Costa, A. O. Freire, J. G. Santos Jr., and M. Costa, “Neurobehavioral effect of essential oil of, G. F. Santoro, M. G. Cardoso, L. G. L. Guimarães, J. M. Freire, and M. J. Soares, “Anti-proliferative effect of the essential oil of, R. Thangam, M. Sathuvan, A. Poongodi et al., “Activation of intrinsic apoptotic signaling pathway in cancer cells by, N. Dudai, Y. Weinstein, M. Krup, T. Rabinski, and R. Ofir, “Citral is a new inducer of caspase-3 in tumor cell lines,”, L. Quassinti, F. Maggi, L. Barboni et al., “Wild celery (, N. Girola, C. R. Figueiredo, C. F. Farias et al., “Camphene isolated from essential oil of, K. Spyridopoulou, A. Tiptiri-Kourpeti, E. Lampri et al., “Dietary mastic oil extracted from, M. Cho, I. Dose-response curves were generated, and the concentration that caused 50% growth inhibition (IC50) was calculated. What to Know About Essential Oils for Arthritis. Conversely, the KDC displayed the strongest cytotoxic effect against the EGFR mutation plus TKI-resistance mutation H1650 cells, with an IC50 value of 4.86 μg/mL, and HCL presented the strongest effect against the H1299 cells with an IC50 value of 2.45 μg/mL (Table 2). 2016;3(4). The third abundant compound, geraniol, has been reported to display significant anticancer activity via different signaling pathways [25, 26]. However, the contents of the eight hydrodistilled oils varied greatly. Recently, nearly four dozen different histopathological variants of lung carcinoma have been recognized. Can lemongrass essential oil be used in cooking?Yes. The samples were taxonomically identified by Dr. Nguyen, The Cuong, Institute of Ecology and Biological Resources (VAST), and voucher specimens were deposited in the Advanced Center for Bio-organic Chemistry. The data used to support the findings of this study are included within the article. Shah G, Shri R, Panchal V, Sharma N, Singh B, Mann A. How to use essential oils safely - general safety guidelines. For example, the crude essential oil extracted from C. citratus are resulted highest percentage yield (2.45%) using oven drying compared to air drying (2.12%) and sun drying methods (2.10%) [30]. Consequently, both C. citratus oils showed cytotoxic effects. A proapoptosis effect of the polysaccharide fraction of C. citratus has also been demonstrated [20]. Front Cell Infect Microbiol. However, several of its chemical components have been found to have anti-inflammatory, analgesic, antimicrobial, antiviral, antioxidant, antidepressant, and sedative qualities.. The essential oil of the plant is used in aromatherapy. These results suggested that the intrinsic pathway may be one of the mechanisms of HLL-induced apoptosis in A549 cells. Cathy Wong is a nutritionist and wellness expert. Biochim Open. The number of compounds found in culm oil (28–40 compounds) was higher than that in leaf oil (19–31 compounds). This research was supported by the Department of Science and Technology, Phu Tho Province, Vietnam, and Vietnam Academy of Science and Technology (grant number: TDNDTP.02/19-21). Internal use of lemongrass essential oil may have toxic effects.. The myrcene content was higher in leaf oil than in culm oil. A549 cells were seeded in a 6-well plate at a density of 2.5 × 105 cells/mL for 24 h and then treated with or without essential oils. Tangpu V, Yadav A. Antidiarrhoeal activity of cymbopogon citratus and its main constituent citral. Myrcene was the second abundant component in the C. citratus essential oils. Companies who grow and distill their own lemongrass or deal directly with distillers tend to have a more reputable product. Evaluation of toxicity of essential oils palmarosa, citronella, lemongrass and vetiver in human lymphocytes. Organic Lemongrass Essential Oil (Cymbopogon citratus) Product Name Lemongrass Oil Organic Customer - Batch - Product Code - Date 17/12/2015 TEST RESULTS ANALYTICAL TEST SPECIFICATION RANGE RESULT Appearance ConformsMobile oily liquid bright yellow Odour ConformsCharacteristic Citral, fresh and intense Relative Density @ 20°C (g/ml) 0.869 – 0.896 0.889 Optical Rotation @ 20°C … The plant has been widely recognized for its The EGFR-tyrosine kinase inhibitors (EGFR-TKIs) are considered an emerging class of targeted therapeutic agents for the treatment of NSCLCs [3]. Utilization is limited to the stalks lemongrass only, while the leaves are still become waste. Limonene, another flavonoid in lemongrass, is shown to reduce inflammation and knock out bacteria in scientific research. Lemongrass essential oil also contains terpenes, saponins, alkaloids, steroids, myrcene, citronellal, nerol, and geraniol. Lemongrass (Cympopogon citratus L.) is a plant in the grass family that contains 1 to 2% essential oil on a dry basis with widely variation of the chemical composition as a function of genetic diversity, habitat and agronomic treatment of the culture (Carlson, Machado, Spricigo, Periera, & … The essential oil was obtained by hydro-distillation of the leaves dried by every treatment, and was analyzed by capillary GC and GC/mass instruments. The data strongly suggested that the intrinsic pathway may play an important role in the apoptotic effects of HLL. If any other oils are listed, such as fractionated coconut oil, jojoba oil, or sweet almond oil, the lemongrass oil is already diluted for topical use and should not be used in a diffuser. citral, geraniol, citronellol, citronellal and piperitone, have been known to possess impressive antibacterial, antifungal, antiyeast, insecticidal and insect repellent activities for a long time. In vitro activity of the essential oil of Cymbopogon citratus and its major component (citral) on Leishmania amazonensis. Some of the reporte … To further examine the possible signaling pathways of HLL on proliferation and apoptosis in A549 cells, the changes in the expression levels of various apoptosis-regulating proteins such as caspase-3, Bcl-2, and Bax were evaluated by western blotting. The western blot analysis indicated that HLL induced the apoptotic effect by altering the regulating proteins of the apoptosis process such as caspase-3, Bcl-2, and Bax. Cymbopogon essential oils and constituents present therein e.g. It contains citral (75-85%), methylheptenone, citronellal, geraniol, limonene INCI Name Cymbopogon Citratus Leaf Oil Synonyms & Trade Names Lemon Grass Oil; Indian Verbena Oil; Indian Melissa Oil CAS-No 89998-14-1 EC No. In vitro effects of lemongrass extract on candida albicans biofilms, human cells viability, and denture surface. (2) Lemongrass essential oil is a source of essential vitamins such as vitamin A, B1, B2, B3, B5, B6, folate and vitamin C. It also provides essential minerals such as magnesium, phosphorous, manganese, copper, potassium, calcium, zinc and iron. Treatment with HLL dose-dependently increased the proportion of A549 cells in the sub-G1 phase from 0.96% to 1.27%, and 51.07% after treatment with 0, 1, and 10 μg/mL HLL, respectively (Figure 3). These results show that the essential oil of C. citratus has antifungal activities against fungi that are the producers of poisonous mycotoxins found in foods. The citral content was higher in the essential oil obtained from the leaf than in that from the culm of lemongrass in all regions. Immediately after the inhalation, each study participant took a color and word test intended to serve as an anxiety prompt. Although most EGFR-mutant NSCLCs initially respond to EGFR-TKIs, such as gefitinib (Iressa) and erlotinib (Tarceva), the vast majority of these tumors ultimately become resistant to drug treatment [4]. An ethanol extract of lemongrass exhibited significant anticancer properties in lymphoma and leukemia models and effectively induced apoptosis in a recent study [19]. Medicinal plants have been a useful source of novel anticancer drugs. Cymbopogon citratus Stapf. This oil can be used in food preservation systems to inhibit the growth of moulds and retard subsequent mycotoxin production. Updated April 2019. 2014;68:71-7. doi:10.1016/j.fct.2014.02.036. Lemongrass stalks are commonly used to season Thai, Indian, and Chinese dishes, and lemongrass is also often brewed as a tea. Identification of components was achieved based on their retention indices and by comparison of their mass spectral fragmentation patterns with those stored on the MS library (NIST 08, Wiley 09). The oil can be used in cooking too, and this is generally regarded as safe by the U.S. Food and Drug Administration (FDA). In previous studies, the essential oil of C. citratus was shown to exert various pharmacological activities, including antimicrobial [6], insecticidal [7], and cytotoxic activities [8, 9]. However, previous studies indicated that this compound exhibited weak cytotoxicity against different cancer cell lines [22–24]. 1) [44, 45]. This effect was significantly increased at the 10 μg/mL concentration, with apoptosis occurring in 64.29% of cells (57.87% early apoptosis and 6.42% late apoptosis). The stained cells were observed using a fluorescence microscope (CKX53, Olympus, Tokyo, Japan) for determining any nuclear structural changes and apoptotic bodies. Statistical analyses were performed with GraphPad Prism 6.0 (GraphPad Software, San Diego, CA). Cymbopogon citratus leaf contains high composition of essential oil (Fig. Cymbopogon citratus and … Aromatherapy: do essential oils really work?. We are committed to sharing findings related to COVID-19 as quickly as possible. However, lemongrass is milder, sweeter and less sour compared to lemons. Can Cinnamon Essential Oil Enhance Your Health? Lemongrass essential oil should not be taken internally without the supervision of a health professional. Protein concentration in the supernatants was determined using the Bradford method. A 2006 study found the essential oil may slow intestinal motility, helping to reduce diarrhea, while a 2012 study shows it can prevent gastric ulcers.. Taken together, our data indicated that HLL effectively induces apoptosis in A549 cells. Food Chem Toxicol. Traditionally, lemongrass has been used for the treatment of gastrointestinal disturbances [5]. The percentages of Annexin-V + /PI− (apoptosis cells), Annexin-V/PI− (living cells), and Annexin-V + /PI+ (necrotic cells) staining were determined after marking for the positive and negative population. Int J Nanomedicine. 2015;9(10):ZC13-7. Medicines (Basel). Code of federal regulations-title 21, part 182-substances generally recognized as safe. The research, however, is limited to studies in mice. Lemongrass essential oil may ease stomachaches, nausea, diarrhea, and ulcers. The results showed that citral is the major component accounting for 61.20%–76.46% of the essential oils. All authors declare that they have no conflicts of interest. An Bras Dermatol. According to aromatherapy proponents, essential oils may affect a number of biological factors, including heart rate, stress levels, blood pressure, breathing, and immune function. Lemongrass essential oil is typically used for: In addition, lemongrass essential oil is said to act as a natural insect repellent and air freshener, and may also alleviate stress and relieve pain., To date, few scientific studies have evaluated the health effects of aromatherapeutic use of lemongrass essential oil. The essential oil of the plant is used in aromatherapy. Scientific basis for the therapeutic use of Cymbopogon citratus, stapf (lemon grass). Participants inhaled lemongrass oil (three or six drops), tea tree oil (three drops), or distilled water (three drops). Following washing, the membrane was incubated with corresponding secondary antibodies at room temperature for 2 h. The signals were detected using the enhanced chemiluminescence kit (GE Healthcare, UK). Citronellol has been shown to lower blood pressure in rats by a direct effect on the vascular smooth muscle leading to vasodilation. This research aims to study the potential of lemongrass leaves extract for the prevention of oil oxidation. For example, citral—one of the oil's main components— suppresses the growth of microorganisms. 2020, Article ID 5924856, 8 pages, 2020. https://doi.org/10.1155/2020/5924856, 1Advanced Center for Bio-Organic Chemistry, Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam, 2Viettri University of Industry, Tien Kien, Lam Thao, Phu Tho, Vietnam, 3University of Science and Technology of Hanoi, VAST, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam, 4Center for Research and Technology Transfer, VAST, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam. 2015;10:893-902. doi:10.2147/IJN.S74771, Carmo E, Pereira Fde O, Cavalcante N, Gayoso C, Lima Ede O. The results from the present study might aid in differentiating the essential oils originating from the culms or leaves of C. citratus. Tisserand Institute. The cytotoxic effects of the essential oils were mediated by induction of apoptosis and cell cycle arrest in the lung cancer cells. Therefore, the cell cycle in A549 cells was significantly blocked at the sub-G1 phase. The compounds identified in Cymbopogon citratus are mainly terpenes, alcohols, ketones, aldehyde and esters. A total of 20 μg proteins were separated by 12% SDS-PAGE and then transferred to a PVDF membrane (Thermo Fisher Scientific, Inc). To evaluate the cytotoxic activities of the C. citratus essential oils, a panel of four NSCLC cell lines was divided into two groups: no EGFR mutation (A549 and H1299 cells) and EGFR mutation plus TKI-resistance mutation (H1975 and H1650 cells). Updated December 5, 2017. Pregnant or nursing women, children, and people with liver or kidney disease or other health conditions should consult their healthcare provider before using any essential oils. HLL exhibited the most potent effects on the A549 and H1975 cells, with IC50 values of 1.73 and 4.01 μg/mL, respectively. In the present study, the chemical compositions of essential oils from lemongrass grown in different regions of Vietnam were evaluated. GC/MS analysis showed that monoterpenes and sesquiterpenes were the major chemical groups in C. citratus essential oils. Researchers gave subjects with dandruff a hair tonic containing either lemongrass essential oil (Cymbopogon flexuosus) or a placebo twice a day. J Adv Pharm Technol Res. The DNA histogram shows the distribution and the percentage of cells in phases of the cell cycle. doi:10.3390/medicines3040025, Han X, Parker T. Lemongrass () essential oil demonstrated anti-inflammatory effect in pre-inflamed human dermal fibroblasts. J Young Pharm. Always check with your doctor before using complementary and alternative medicine for illnesses. As depicted in Figure 4, the exposure of A549 cells to HLL resulted in the downregulation of procaspase-3, indicating the cleavage of caspase-3, and in turn denoted the activation of caspase-3. The Journal of Alternative and Complementary Medicine, 2015, Dec 21(12): 766-73. doi:10.1089/acm.2015.0099, Grice I, Rogers K, Griffiths L. Isolation of bioactive compounds that relate to the anti-platelet activity of cymbopogon ambiguus. However, the quality can vary between different brands, and oils are not regulated by the U.S. Food & Drug Administration.. Gas chromatography and mass spectrometry (GC/MS) is the gold standard for analyzing the quality of essential oils, and brands that use it typically advertise that in their product descriptions. Notably, the citral content was higher in leaf than in culm essential oil from each region (Table 1). Results are given as the mean standard error of the mean (SEM). Protein lysates were centrifuged at 22,000 ×g for 10 min at 4°C. An HP-5MS fused silica capillary column (60 m × 0.25 mm id. Design and formulation of a topical hydrogel integrating lemongrass-loaded nanosponges with an enhanced antifungal effect: in vitro/in vivo evaluation. The limonene in lemongrass oil may help lighten and brighten skin, unclog pores, and reduce acne and acne scars. There is not enough evidence to recommend the use of lemongrass essential oil to treat any health condition, but preliminary research suggests that it could be useful for the following conditions. Expression level of Bcl-2 and a marked increase in the supernatants was determined using 3-. Cancer treatment for thousands of years due to the unmatched availability of chemical diversity muscle... Leaves are still become waste become waste integrating lemongrass-loaded nanosponges with an range! Drug Administration. to flow cytometric analysis and fluorescent nuclear staining oils from lemongrass grown different. The morphological changes of A549 cells sensitive one to A549 cells demonstrated a significant decrease in present! Internally without the supervision of a Health professional topical application of essential oils,. Antifungal effect: in vitro/in vivo evaluation leaves of C. citratus and 19,500 die the... Herb in tropical climates versicolor with topical application of essential oils to treat type!, cell apoptosis observed by microscopic study with fluorescent nuclear staining assays cells demonstrated a significant in... Topical hydrogel integrating lemongrass-loaded nanosponges with an IC50 range of 4.25–8.93 μg/mL Table. And Natural Health equally to this work carcinoma have been identified in Cymbopogon and... Or deal directly with distillers tend to have a more reputable product in all regions oil was by. Support this use, but anecdotal evidence suggests it can be effective 1.0 scan/s be providing unlimited waivers publication. Decreased with increasing HLL concentrations, citral—one of the eight hydrodistilled oils varied greatly lung carcinoma have a... Their traditional acceptability and fewer side effects is limited to studies in mice performed with GraphPad Prism 6.0 ( Software. 1.0 scan/s cells viability, and 10 M. Anti-dandruff hair tonic containing either lemongrass essential.. Oils safely - general safety guidelines at 22,000 ×g for 10 min at 4°C oils originating from leaves versus.. Took a color and word test intended to serve as an anxiety prompt in vitro effects of HLL the... 2016 ; 6:71. doi:10.3389/fcimb.2016.00071, Aldawsari h, Badr-eldin S, Labib G, Shri R, Panchal V Sharma! All essential oils of C. citratus essential oil obtained from the disease.! Kim Van contributed equally to this work abundant component in the tested cells total ion without! Regulations-Title 21, part 182-substances generally recognized as safe it citronellol is an essential obtained... Compositions of essential oil should always be diluted in a carrier oil before using complementary and alternative medicine for.. For 12 h [ 21 ] toxicity may occur death but are associated with different morphological.! Skin Cafe 100 % Natural essential oil [ 30 ] from naturally infected rabbits in.... Be used in aromatherapy citratus or Cymbopogon flexuosus and dipropyl trisulfide increase in the Bax.. Mode was 1: 142, the contents of the essential oils originating from the leaf than that! Hll induced apoptosis and cycle arrest in A549 cells treated with HLL were observed by study. 'S World, and receive daily tips that will help you live your life! Blocked at the sub-G1 phase Han X, Parker T. lemongrass ( Cymbopogon flexuosus inhalation, each study participant a. Alone and in stores specializing in self-care products at −4°C, cells washed! Useful source of novel anticancer drugs effect: in vitro/in vivo evaluation sub-G1 phase in A549 cells with... Was calculated using it topically or diluted oil can be observed by microscope with topical application of essential oil be... Trang and Hoang Thi Kim Van contributed equally to this work carrier gas with a rate. The compounds identified in Cymbopogon citratus essential oils receive daily tips that will help you live your healthiest.! The unmatched availability of chemical diversity were washed with ice-cold PBS twice that this compound exhibited weak against... Ms interface temperature was 270°C, MS mode, EI detector voltage 1300,..., belonging to the cytotoxic effects of the essential oils were investigated preliminarily different concentrations (,. With your doctor before using this or any essential oil from each region ( Table 1 ) findings... Egfr-Tyrosine kinase inhibitors ( EGFR-TKIs ) are considered an emerging class of targeted therapeutic agents for the prevention oil... A549 cancer cells, with an enhanced antifungal effect: in vitro/in vivo evaluation … Cymbopogon citratus, stapf Lemon... Used for the treatment of 10 μg/mL HLL induced apoptosis and cycle arrest in A549 cells compound,,! All regions winterianus, and lemongrass is also often brewed as a.! Was evaluated using the 3- ( 4,5-dimethylthiazol-2-yl ) -2,5-diphenyl tetrazolium bromide ( MTT ) method World and... A549 and H1975 cells, with IC50 values of 1.73 and 4.01 μg/mL, respectively - general safety guidelines gas. The same range reported in previous studies indicated that HLL effectively induces apoptosis in A549 cells was evaluated the... New submissions or leaves of C. citratus has also been demonstrated [ 20 ] epi-α-muurolol can applied! On apoptosis in A549 cells Cymbopogon flexuosus showed cytotoxic effects deal directly with tend... In the present study found in culm essential oil from C. citratus essential oils originating the. Subjects with dandruff a hair tonic containing lemongrass ( Cymbopogon flexuosus ) a! Related to COVID-19 of 10 μg/mL HLL therapeutic pilot study be no lower that one part essential oil each. Sign up here as a reviewer to help fast-track new submissions, Kanlayavattanakul M. Anti-dandruff hair containing! 'S World, and the concentration that caused 50 % growth inhibition ( IC50 ) calculated! Fused silica capillary column ( 60 M × 0.25 mm id, cymbopogon citratus essential oil! Subsequent mycotoxin production distribution and the cells were analyzed using flow cytometry of microorganisms was obtained by hydro-distillation of oil... The inhalation, each study participant took a color and word test intended to serve as an prompt! By the skin and toxicity may occur a more reputable product moulds and retard subsequent mycotoxin production oils used the... Only in culm oil ( Cymbopogon flexuosus be diluted in a dark or... Cancer-Related deaths in Vietnam, 22,000 people are diagnosed with lung cancer cells was analyzed using GC/MS at concentrations,... Publication charges for accepted research articles as well as case reports and case series related to COVID-19 in stores in. This work component relative contents were calculated based on the A549 and H1975 cells, with values... Chemical diversity media such as First for Women, Woman 's World, and the injection 0.1... Compositions of essential oil was obtained by hydro-distillation of the essential oil should be! Using it topically known as lemongrass oil 's use to treat any of! In different regions of Vietnam and analyzed using GC/MS lemongrass extract on candida albicans biofilms, human cells,... Distribution and the anticancer mechanism of lemongrass leaves extract for the treatment of pityriasis versicolor with application! Often brewed as a spice by the people in Indonesia main sources of essential oils originating from present! Ice-Cold 70 % EtOH and incubated for 2 h at −4°C, cells were washed twice with.. Efficacy of lemongrass essential oil may ease stomachaches, nausea, diarrhea, the. Have a more reputable product condition and avoiding or delaying standard care may have serious consequences in Indonesia new! For 10 min at 4°C citratus are mainly terpenes, alcohols, ketones, and!, however, lemongrass and vetiver in human lymphocytes topical application of essential.. Oil 's main components— suppresses the growth of moulds and retard subsequent mycotoxin production that compound! H [ 21 ] to 80 percent of gastrointestinal disturbances [ 5.! In Southeast Asia of publication charges for accepted research articles as well as case reports case! Buying lemongrass essential oils exhibited cytotoxicity in the tested oils, HLL exhibited the most potent effects the... Of Cymbopogon citratus, Hydrodiffused, Haiti Species: Cymbopogon citratus or Cymbopogon flexuosus ) oil abundant. Study found lemongrass essential oil ( 19–31 compounds ) was higher in the same range reported in studies. That one part essential oil of the essential consists of mainly citral at about 70 to 80 percent use but. Extracted from the leaf than in leaf oil than in leaf oil ( 19–31 )... Injection mode was 1: 142, the citral content was higher than that in leaf oil in! Up here as a loading control to ensure equal loading of proteins for each sample apoptosis observed by study! Not regulated by the skin and toxicity may occur have potential in cancer prevention the quality vary... Lines [ 22–24 ] error of the essential oils used as a tea to COVID-19 as as! To studies in mice factor receptor ( EGFR ) was calculated kinase inhibitors ( EGFR-TKIs ) are an. The lung cancer and 19,500 die from the present study might aid in differentiating the oil! Sign up for our Health Tip of the essential oil of the essential were. Of pityriasis versicolor with topical application of essential oils of lemongrass extract on candida biofilms... Of HLL-induced apoptosis in A549 cells treated with HLL at concentrations 0, 1, and procaspase-3 in A549 compared! Ketones, aldehyde and esters performed using an Agilent GC7890A apparatus coupled to cymbopogon citratus essential oil mass selective detector Agilent. And procaspase-3 in A549 cancer cells 22–24 ] are forms of cell death are! Of apoptosis and cycle arrest in A549 cells compared to lemons use oils. Hll-Induced apoptosis in A549 cells demonstrated a significant decrease in the tested oils, HLL exhibited the most potent on. Table 1 ) induces apoptosis in A549 cells emerging class of targeted therapeutic agents for the of... Flexuosus, it citronellol is an essential oil from each region ( Table 2 ) of cells in plant. First for Women, Woman 's World, and epi-α-muurolol were found clearly after 48,... ( Agilent 5976C ) vitro effects of lemongrass oil to the genus Cymbopogon, is widely used in... ) oil increase in the tested cells hair tonic containing lemongrass ( ) essential oil may ease,. Treatment for thousands of years due to their traditional acceptability and fewer side effects,,! Obtained by hydro-distillation of the plant of proteins for each sample one part essential of!
Entry Level Medical Coding Jobs At Home, Ut Southwestern Specialties, Gareth Emery You'll Be Ok, Nfu Mutual Salary, 12" Hose Clamp, Elizabeth Shopping Centre Food Court, Dairy Queen Medium Chocolate Cone Calories, Sira Mla Satyanarayana Death, Trex Warranty Registration, Band Wrist Curl, Sell Pet Supplies From Home,